11965DMEM培养基的详细资料:
DMEM培养基是一种含各种氨基酸和葡萄糖的培养基,是在MEM培养基的基础上研制的。与MEM比较增加了各种成分用量,同时又分为高糖型(低于4500mg/L)和低糖型(低于1000mg/L)。高糖型有利于细胞停泊于一个位置生长,适于生长较快、附着较困难肿瘤细胞等。
所以其特点主要包括以下:
(1)氨基酸含量为依格尔的2倍,且含有非必需氨基酸,如甘氨酸等;
(2)维生素含量为依格尔的4倍;
(3)含有糖酵解途径中的重要物质——丙酮酸;
(4)含有微量的铁离子。
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